cellxvivo human treg cell differentiation kit  (R&D Systems)

Journal: Immunologic research

Article Title: Staphylococcus aureus α-hemolysin induces DNA methylation changes in human Th1 cells.

doi: 10.1007/s12026-025-09647-0

Figure Lengend Snippet: Fig. 1 The impact of α-hemolysin on the viability of CD4+ T cells differentiating into human Th1 lymphocytes was investigated. CD4+ cells from six different donors were exposed to increasing con- centrations of α-hemolysin and differentiated into Th1 lymphocytes over a period of five days. Cell viability was then assessed via the CellTiter-Glo® Luminescent Cell Viability Assay (Promega). The results are presented as statistical dot plots, with median values indi- cated by bars (n = 6); * indicates statistical significance at p < 0.05

Article Snippet: These cells were then differentiated into Th1 cells over a 5-day period via a Human Th1 Cell Differentiation Kit (R&D Systems, CDK001, Minneapolis, MN, USA) following the manufacturer’s protocol.

Techniques: Cell Viability Assay

Journal: Immunologic research

Article Title: Staphylococcus aureus α-hemolysin induces DNA methylation changes in human Th1 cells.

doi: 10.1007/s12026-025-09647-0

Figure Lengend Snippet: Fig. 10 Ratio of DMRs in gene functional regions modified by CGs, CHGs, and CHHs in Th1 lymphocytes

Article Snippet: These cells were then differentiated into Th1 cells over a 5-day period via a Human Th1 Cell Differentiation Kit (R&D Systems, CDK001, Minneapolis, MN, USA) following the manufacturer’s protocol.

Techniques: Functional Assay, Modification

Journal: International Journal of Molecular Sciences

Article Title: Paeoniflorin Directly Targets ENO1 to Inhibit M1 Polarization of Microglia/Macrophages and Ameliorates EAE Disease

doi: 10.3390/ijms26083677

Figure Lengend Snippet: Figure 3. PF reduces the percentages of M1 microglia/macrophages and pathogenic T cells in the CNS of EAE mice. The CNS mononuclear cells were isolated from the EAE-PBS and EAE-PF group mice on day 16 post immunization. (A,B) Flow cytometry analysis of CD45int CD11b+ microglia cells and CD45high CD11b+ macrophages in CNS from the two group mice (n = 4). (C) Flow cytometry analysis of CD86+ or CD206+ cells in CD11b+ cells from the two group mice (n = 4). (D) The mRNA expression levels of cytokines and chemokines in CNS mononuclear cells from the two group mice were determined by qPCR (n = 4). (E) The percentage of CD4+ T cells and T-helper cells such as Th1, Th17, and Treg cells in the CNS were analyzed by flow cytometry (n = 4). Data are expressed as the mean ± SEM. * p < 0.05, ** p < 0.01, **** p < 0.0001; ns, no significance.

Article Snippet: For T cell differentiation, 2 × 105 CD4+ T cells were cultured with corresponding medium for 72 h: Th1 differentiation medium was supplemented with the same concentration of anti-CD3e/CD28 mAbs, anti-IL-4 (10 μg/mL), IL-12 (10 ng/mL), as well as IL-2 (10 ng/mL); Th17 differentiation media were mixed according to the CellXVivo Th17 Cell Differentiation Kit (R&D Systems, Inc., Minneapolis, MN, USA); for Treg cell differentiation, the same concentration of anti-CD3e/CD28 mAbs, anti-IL-4 (10 μg/mL), and anti-IFN-γ (10 μg/mL), TGF-β (15 ng/mL), IL-2 (10 ng/mL) were included.

Techniques: Isolation, Flow Cytometry, Expressing